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ea0073aep405 | Environmental Endocrinology | ECE2021

Development of in vitro rat, dog and human deiodinase inhibition assays with LC-MS/MS analysis for the identification of endocrine disruptors

Lee Dongtao , Macleod Kenneth , Higgins Larry , McBrinn Rachel , Dickinson Robin , McGinnis Claudia

Thyroid hormones are important regulators of metabolism and development. Iodothyronine selenodeiodinases can both activate and inactivate thyroid hormones (Bianco et al. 2002) thus in vitro assays to quantify deiodinase inhibition are considered to be a valuable tool for studying effects on thyroid hormone metabolism by potential endocrine disruptors. The prohormone thyroxine (T4) is converted to 3, 5, 3-triiodothyronine (T3) by outer-ring deiodination,...

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ea0081ep625 | General Endocrinology | ECE2022

The development of a GLP protocol for the measurement of 17β-estradiol and testosterone in the H295R steroidogenesis assay, Test No 456

Murray Carol , McIntyre Angela , McKeever Connor , White Rebecca , McBrinn Rachel , Light Victoria , Brown Susan , Robinson Aron , Clark Graeme , Bingham Tilly

Regulators are concerned about the potential for environmental chemicals such as agrochemicals and their metabolites to perturb hormone systems. This has led to recommendations for the testing of potential endocrine disrupting chemicals1. The Steroidogenesis H295R assay is an in vitro cell model used to investigate compound effects on steroid hormone biosynthesis, specifically 17β-estradiol (E2) and testosterone (T). The human H295R adreno-carcinoma ce...

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Endocrine Abstracts

Development of in vitro rat, dog and human deiodinase inhibition assays with LC-MS/MS analysis for the identification of endocrine disruptors

The development of a GLP protocol for the measurement of 17β-estradiol and testosterone in the H295R steroidogenesis assay, Test No 456

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